Metabolomic facility

Gas chromatography-coupled mass spectrometry (GC-MS

Gas chromatography-coupled mass-spectrometer (GC-MS).

Manufacturer: Chromatec (Russia), model: Crystal 5000

Current protocol quantifies:

  • proteinogenic amino acids (except arginine)
  • non-proteinogenic amino acids (ornithine, cystine, cystathionine)
  • glycolysis intermediates
  • TCA cycle metabolites
  • sugars (glycose, fructose, sucrose)
  • nucleic basis and nucleosides
  • m-inositol, niacin, taurine, cholesterol, phosphoglycerol, glycerol
  • fatty acids

The protocol of sample preparation can be found in O. Fiehn (Curr. Protoc. Mol. Biol., 2016).